Composite

Part:BBa_K936016:Design

Designed by: Pui Yan Ho   Group: iGEM12_UC_Davis   (2012-09-07)

J23101+B0034+Reductase (anaerobic)+B0034+Dehydrogenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 694
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1472
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 944
    Illegal AgeI site found at 1145
    Illegal AgeI site found at 1744
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reductase and Dehydrogenase was isolated from e. coli MG1655, and ligated with the other biobrick parts (promoter and RBS)

Source

E. coli MG1655

References